The cobalt(II)-alkaline phosphatase system at alkaline pH.
نویسندگان
چکیده
منابع مشابه
Alkaline phosphatase activity and pH optima.
High pH values have been found necessary for optimum activity of alkaline phosphatase in vitro. The pH values in the living cell structures, on the other hand, are near neutrality: cytoplasm 6.9, nucleus 7.6 (l-3). This definite difference between the pH required for optimum activity and the pH of the living cell indicates that alkaline phosphatase is not optimally active in viva. However, the ...
متن کاملFluoride and Alkaline Phosphatase
Since serum alkaline phosphatase increases in fluoride therapy for osteoporosis, it is generally accepted that fluoride stimulates bone formation. However, histochemical studies have shown that alkaline phosphatase is also increased in resorbing osteocytes. Fluoride is toxic to metabolically active bone cells, alkaline phosphatase is released, and serum alkaline phosphatase increases. We propos...
متن کاملMicrotubule assembly and disassembly at alkaline pH
Although it is now apparent that the intracellular pH may rise considerably above neutrality under physiological conditions, information on the effect of alkaline pH on microtubule assembly and disassembly is still quite fragmentay. We have studied the assembly/disassembly of bovine brain microtubule protein at alkaline pH in vitro. When microtubules are assembled to a new steady state at pH le...
متن کاملChicken Intestinal Alkaline Phosphatase
Purified chicken intestinal alkaline phosphatase is active at pH 8 to 9, but becomes rapidly inactivated with change of pH to 6 or less. Also, a solution of the inactivated enzyme at pH 4.5 rapidly regains its activity at pH 8. In the range of pH 6 to 8 a solution of purified alkaline phosphatase consists of a mixture of active and inactive enzyme in equilibrium with each other. The rate of ina...
متن کاملAlkaline Phosphatase of Milk
inor i8obutanol. Removal of the lipid is accompanied by simultaneous release of the enzyme into true solution. No other organic solvent tested was effective in releasing the bound phosphatase, indicating a unique action of butanol. I wish to thank Dr M. Dixon, F.R.S., for his encouraging interest and advice during this work. This work was carried out while holding a Travelling Scholarship award...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)37951-1